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Dr Sharon Sagnella PDF Print E-mail

 

 QUALIFICATION:

 


  • Case Western Reserve University, Cleveland, OH, USA
  • PhD Biomedical Engineering May 2003; Area: Cardiovascular Biomaterials/ Tissue Engineering
  • MS Biomedical Engineering January 2000; Area: Cardiovascular Biomaterials
  • BS Degree May 1998 - Major: Biomedical Engineering; Minor: Chemistry

 

 

RESEARCH EXPERIENCE:


Post Doctoral Fellow/Research Scientist – Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia - January 2005 – February 2011.

Research ProjectDevelopment of Self Assembled Endogenous Surfactants for Drug Delivery Applications: Designed and synthesised endogenous ethanolamide amphiphiles and derivatives.  Characterisation and purification of molecules using TLC, HPLC, LC-MS, and NMR.  Examination of physicochemical properties of surfactants using DSC, TGA, cross polarised optical microscopy, XRD, and phase behavior studies.  Utilisation of SAXS for characterisation of mesophases.  Fabrication and characterisation of self-assembled nanoparticle dispersions for drug delivery purposes from bulk mesophases via DLS, SAXS/synchrotron SAXS and Cryo-TEM.  Examination of in vitro and in vivo toxicity.  Examination of the delivery of nanoparticles and chemotherapeutic produgs in vitro and in vivoIn vivo involved a variety of mouse tumour models including HuH (liver cancer, xenograft), MDA-MB-231 (Breast Cancer, xenograft), 4T1 (breast cancer, autograft) and the following techniques:  IP Injections, orogastric gavage, dissection and organ collection, cardiac puncture, tumour inoculation, tail/saphenous vein blood collection.

 

Research Scientist - National Metal and Materials Technology Center, Pathumthani, Thailand - October 2003 – April 2004.

Research Projects: Development of novel biomimetic composite scaffolds to promote osteogensis: Assist in the design and synthesis of peptide coated hydroxyapatite surfaces to serve as an osteogenic material.  Utilised cell culture techniques, confocal microscopy, SEM, biochemical assays for proliferation and alkaline phosphatase activity to assess osteoblast adhesion, growth, and differentiation.

Development of novel biomimetic surfactant polymers to promote endothelial cell adhesion and growth: Design and synthesis of peptide surfactant polymers with a Chitosan backbone to facilitate endothelial cell adhesion and growth.

 

 

Post Doctoral Position – Cleveland Clinic Foundation/ CWRU, Cleveland, OH - May 2003 – September 2003. 

Research Projects: Liposomal Drug Delivery – Examined peptide inhibition of platelet activation and aggregation via platelet aggregometry; Utilised flow cytometry, fluorescence microscopy and platelet aggregometry to analyse peptide modified liposome binding to activated and unactivated platelets.

Fluorosurfactant Polymers designed for Improved Blood Compatibility: Studied blood compatibility of fluorosurfactant modified Teflon.  Implemented rotating disk apparatus to perform hemodynamic testing of platelet activation and adhesion to modified surfaces.   

 

 

Cleveland Clinic Foundation- Hemostasis and Thrombosis Laboratory/ CWRU Biomaterials Laboratory, Cleveland, OH  - May 1996 – May 2003 Advisors: Dr Roger Marchant, Dr Kandice Kottke-Marchant.

PhD Dissertation: Endothelial Cell Adhesion, Growth and Function on Biomimetic Surfactant Polymers - Collaborative development of extracellular matrix like materials to improve EC growth, promote EC migration, and improve EC shear stability; Designed and implemented hemodynamic testing systems to examine EC behavior under flow; Utilised cell culture techniques, fluorescent antibody and cytoskeleton labeling, optical microscopy, fluorescence microscopy, confocal microscopy, proliferation assays to assess success of EC adhesion, growth, migration and shear stability; Discovered increasing surface peptide density altered EC migration and improved EC shear stability.

 

Master’s Thesis: Evaluation of the effect of endothelial cell seeding on platelet activation and adhesion by plasma polymer surface. Synthesised self-assembled monolayers and plasma polymer films; Evaluated platelet activation and adhesion on these materials using blood preparation techniques, fluorescence activated flow cytometry, ELISA assays, fluorescence microscopy; Discovered hydrophilicity promotes platelet activation and adhesion; Incorporated EC monolayer and improved material hemocompatibility.


PATENTS:

D.1 Amphiphile Prodrugs – PCT Ref: M81653596 (4 December 2009)

D.2 Self Assembled Ethanolamides – Provisional Patent Ref: M81371181 (30 March 2009)


 

      HONOURS AND AWARDS:

Whitaker Summer Fellowship (UG), Case Alumni Scholarship, Graduate Trainee Fellowship recipient, Honorable Mention award at Society for Biomaterials Meeting 2002, Travel awardee 2000 and 2002 for New Jersey Biomaterials Symposium; Society for Biomaterials PhD Student award for outstanding research 2003.

 

 

PUBLICATIONS:

A Publications

 

A.1 Sharon Sagnella, Charlotte E. Conn, Irena Krodkiewska, and Calum J Drummond “Nonionic Diethanolamide Amphiphiles with Isoprenoid-Type Hydrocarbon Chains:  Thermotropic and Lyotropic Liquid Crystalline Phase Behavior.”  Physical Chemistry Chemical Physics (submitted) 2011.  

A.2 Gong, X.; Moghaddam, M.; Sagnella,  S. M.; Conn, C. E.; Mulet, X.;  Danon, S.;  Waddington, L. J.;  Drummond, C. J., “Nanostructured Self-Assembly Materials from Neat and Aqueous Solutions of C18 Lipid Pro-drug Analogues of Capecitabine – a Chemotherapy Agent.  Focus on Nanoparticulate CubosomesTM of the Oleyl Analogue”  Soft Matter (Submitted) 2011.

 

A.3 Sharon Sagnella, Charlotte E. Conn, Irena Krodkiewska, and Calum J Drummond “Nonionic Diethanolamide Amphiphiles with Unsaturated C18 Hydrocarbon Chains:  Thermotropic and Lyotropic Liquid Crystalline Phase Behavior.”  Physical Chemistry Chemical Physics (submitted) 2011.  

A.4 Gong, X.; Moghaddam, M.; Sagnella,  S. M.; Conn, C. E.; Danon, S.;  Waddington, L. J.;  Drummond, C. J., “Lyotropic Liquid Crystalline Self-Assembly Material Behavior and Nanoparticulate Dispersions of a Phytanyl Pro-Drug Analogue of Capecitabine – A Chemotherapy Agent”  Applied Materials and Interfaces (in press) 2011.

 

A.5 Sharon Sagnella, Charlotte E. Conn, Irena Krodkiewska, Xavier Mulet, and Calum J Drummond “Anandamide and analogous endocannabinoids: a lipid selfassembly study.”  Soft Matter (in press) 2011.

 

A.6 Gong, X.; Moghaddam, M.; Sagnella,  S. M.; Conn, C. E.; Danon, S.;  Waddington, L. J.;  Drummond, C. J., “Lamellar Crystalline Self-Assembly Behaviour and Solid Lipid Nanoparticles of a Palmityl Pro-Drug Analogue of Capecitabine – A Chemotherapy Agent”  Colloids and Surfaces B (in press) 2011.

 

A.7 Sagnella, S. M.; Gong, X.; Moghaddam, M.; Conn, C. E.; Kimpton, K.; Waddington, L. J.; Drummond, C. J., “Nanostructured Nanoparticles of Self-Assembled Lipid Pro-Drugs as a Route to Improved Chemotherapeutic Agents.” Nanoscale 2011 3: p.919

A.8 Fong, Celesta; Weerawardena, Asoka; Sagnella, Sharon; Mulet, Xavier; Krodkiewska, Irena; Chong, Josephine; Drummond, Calum; "Monodisperse Nonionic Isoprenoid-Type Hexahydrofarnesyl Ethylene Oxide Surfactants: High Throughput Lyotropic Liquid Crystalline Phase Determination" Soft Matter 2011 27; p. 2317.

A.9 Conn, C. E.; Mulet, X.; Moghaddam, M.; Darmanin, C.; Sagnella, S. M.; Kirby, N.; Varghese, J. N.; Drummond, C. J., “Enhanced uptake of an integral membrane protein, the dopamine D2L receptor, by cubic nanoparticles doped with Ni chelated EDTA amphiphiles” Soft Matter 2011 7: p. 567.

 

 

A.10 Conn, C. E.; Darmanin, C.; Sagnella, S. M.; Mulet, X.; Greaves, T. L.; Varghese, J. N.; Drummond, C. J., “Incorporation of the dopamine D2L receptor and bacteriorhodopsin within bicontinuous cubic lipid phases.  1. Relevance to in meso crystallization of integral membrane proteins in monoolein systems” Soft Matter 2010 6: p.4828.

 

A.11 Conn, C. E.; Darmanin, C.; Sagnella, S. M.; Mulet, X.; Greaves, T. L.; Varghese, J. N.; Drummond, C. J., “Incorporation of the dopamine D2L receptor and bacteriorhodopsin within bicontinuous cubic lipid phases.  2. Relevance to in meso crystallization of integral membrane proteins in novel lipid systems” Soft Matter 2010 6: p.4838.

 

A.12 Fong, Celesta; Weerawardena, Asoka; Sagnella, Sharon; Mulet, Xavier; Waddington, Lynne; Krodkiewska. Irena; and Drummon,. Calum J. “Monodisperse Nonionic Phytanyl Ethylene Oxide Surfactants: High Throughput Lyotropic Liquid Crystalline Phase Determination and the Formation of Liposomes, Hexosomes and Cubosomes.”  Soft Matter 2010 6: p4727.

 

A.13    Sharon Sagnella, Charlotte E. Conn, Irena Krodkiewska, and Calum J Drummond “Ordered Nanostructured Amphiphile Self-Assembly Materials from Endogenous Nonionic Unsaturated Monoethanolamide Lipids in Water.”  Langmuir 2010 26(5): p.3084-3094.

 

A.14  Sharon Sagnella, Charlotte E. Conn, Irena Krodkiewska, Minoo Moghaddam, and Calum J Drummond “Endogenous Nonionic Saturated Monoethanolamide Lipids: Solid State, Lyotropic Liquid Crystalline, and Solid Lipid Nanoparticle Dispersion Behaviour.”  Journal of Physical Chemistry B 2010.  114(4).  p.1729-1737.

 

A.15 Sharon Sagnella, Charlotte E. Conn, Irena Krodkiewska, and Calum J Drummond “Soft ordered mesoporous materials from nonionic isoprenoid-type monoethanolamide amphiphiles self-assembled in water.” Soft Matter 2009.  5(23).  p.4823-4834.

 

A.16 Gong XJ, Sagnella S, and Drummond CJ “Nanostructured self-assembly materials formed by non-ionic urea surfactants.”  International Journal of Nanotechnology 2008.  5(2-3): p.370-392.  Impact factor = 1.234  No. of citations = 4 ARC Ranking = C

 

A.17 Wang S, Gupta AS, Sagnella S, Barendt PM, Kottke-Marchant K, and Marchant RE “Biomimetic Fluorocarbon Surfactant Polymers Reduce Platelet Adhesion on PTFE/ePTFE Surfaces.” Journal of Biomaterials Science Polymer Edition 2009.  20(5): p.619-635.

 

A.18 Sharon Sagnella and Katanchalee Mai-Ngam “Chitosan Based Surfactant Polymers Designed to Improve Blood Compatibility on Biomaterials.”  Colloids and Surfaces B-Biointerfaces 2005. 42(2): p.147-155.

 

A.19 S. Sagnella, E. Anderson, R. Marchant, K. Kottke-Marchant, “Biomimetic Surfactant Polymers

Containing Heparin Binding Peptides for Vascular EngineeringTissue Engineering 2005.  11(1-2): p.226-236.

 

A.20 Sen Gupta A, Huang G,  Lestini BJ, Sagnella S, Kottke-Marchant K, Marchant RE  “RGD-modified liposomes targeted to activated platelets as a potential vascular drug delivery system.” Thrombosis and Haemostasis 2005.  93(1): p.106-114.

 

A.21 Mai-Ngam K, Seetapan N, Sagnella S “Effect of Differences in Physical and Chemical Properties of Chitosan on Endothelial Cell Adhesion and Growth” The Medical Journal of Malaysia 2004. 59Suppl B: 172-173.

 

A.22 S. Sagnella, F. Kligman, E. Anderson, J. King, G. Murugesan, R. Marchant, K. Kottke-Marchant “Human Microvascular Endothelial Cell Migration on Biomimetic Surfactant Polymers.” Biomaterials, 2004.  25(7-8): p.1249-1259

 

A.23 S. Sagnella, F. Kligman, R. Marchant, K. Kottke-Marchant “Biomimetic Surfactant Polymers Designed for Shear Stable Endothelialization.” Journal of Biomedical Materials Research, 2003. 67A(3): p.689-701 - (Winner of the 2003 Society for Biomaterials student award for outstanding research in the PhD Category).

 

A.24 Brian J. Lestini, Zhong Xu, Matthew S. Shive, Johnsamuel Jayaseheran, Nancy J. Richter, Sharon M. Sagnella, Aubrey J. Case, Kandice Kottke-Marchant, James M. Anderson, and Roger E. Marchant “Surface Modification of Liposomes for Selective Cell Targeting in Cardiovascular Drug Delivery.” Journal of Controlled Release 2001. 78(1-3):p.235-247.

 

A.25 Sharon Sagnella, Jonathan Kwok, Roger E. Marchant, Kandice Kottke-Marchant “Shear Induced Platelet Activation and Adhesion on Human Pulmonary Artery Endothelial Cell Seeded Hydrophilic Polymers.” Journal of Biomedical Materials Research, 2001. 57(3): p.419-431.

 

Abstracts/Presentations

 

B.1  Sharon Sagnella, Charlotte Conn, Nelly Gong, Irena Krodkiewska, Minoo Moghaddam, Kathleen Kimpton, Stephen Danon, Lynne Waddington, and Calum Drummond “Nano-assemblies as Therapuetic Delivery Agents”  CSIRO Advanced Materials Conference and Workshops, Melbourne, Australia, 2010

B.2 Sharon Sagnella, Charlotte Conn, Nelly Gong, Irena Krodkiewska, Minoo Moghaddam, Kathleen Kimpton, Stephen Danon, Lynne Waddington, and Calum Drummond “Self-Assembled Nanostructured Dispersions as Therapuetic Delivery Agents”  International Conference on Nanoscience and Nanotechnology, Sydney, Australia, 2010

 

B.3 Sharon Sagnella, Irena Krodkiewska, and Calum J Drummond “Nonionic Amide and Monoethanolamide Surfactants with Isoprenoid-Type Hydrocarbon Chains: Thermotropic and Lyotropic Liquid Crystalline Phase Behaviour”  Australian Colloid and Interface Symposium, Programme and Abstracts, Coogee Beach, Australia, 2007

 

B.4 Sagnella S, Anderson E, Sanabria N, Marchant R, Kottke-Marchant K “Biomimcry of the Extracellular Matrix For Endothelialization” Australian Colloid and Interface Symposium, Programme and Abstracts, Coogee Beach, Australia, 2005

 

 


     

     

     

     

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    Contact Details:

    Australian Centre for NanoMedicine

    The University of New South Wales

    SYDNEY NSW 2052

    Australia

    P: +61 0432 990 049

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